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In contrast, embryonic stem cell (ESC) progeny readily yield region-specific neuronal fates in response to appropriate developmental signals.

Here we demonstrate prospective and clonal isolation of neural rosette cells (termed R-NSCs), a novel NSC type with broad differentiation potential toward CNS and PNS fates and capable of in vivo engraftment.

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Mouse models with humanised immune systems represent ground-breaking platforms to evaluate compounds to treat a variety of human diseases, from cancer and infectious diseases to allergies, inflammation and Graft versus Host Disease.

The hosts for hu-CD34 and hu-PBMC models are NOD gamma (NSG™) mouse, developed by JAX Professor Lenny Shultz and the NSG™-SGM3 strain, shown to support greater engraftment of human haematopoietic stem cells (hu-CD34 cells) than all other strains.

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Maintenance of R-NSCs is promoted by activation of SHH and Notch pathways.

In the absence of these signals, R-NSCs rapidly lose rosette organization and progress to a more restricted NSC stage.

Large cohorts of humanised CD34 NSG™ and NSG™-SGM3 mice are available for immediate delivery or to enrol in JAX drug efficacy testing services.

Humanised PBMC (hu-PBMC) mice are used as models to study and evaluate compounds for infectious diseases and graft rejection research.

We propose that R-NSCs represent the first characterized NSC stage capable of responding to patterning cues that direct differentiation toward region-specific neuronal fates.

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